Genome Integrity

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 7  |  Issue : 1  |  Page : 2-

Calibration curve for dicentric chromosomes induced in human blood lymphocytes exposed to gamma rays at a dose rate of 12.5 mgy/s


Tran Que, Pham Ngoc Duy, Bui Thi Kim Luyen 
 Department of Biotechnology, Biodosimetry Section, Nuclear Research Institute, Vietnam Atomic Energy Commission, VINATOM, Dalat, Vietnam

Correspondence Address:
Tran Que
Department of Biotechnology, Biodosimetry Section, Nuclear Research Institute, Vietnam Atomic Energy Commission, VINATOM, No. 1, Nguyen Tu Luc, Dalat
Vietnam

To develop a calibration curve for induction of dicentric chromosomes by radiation, we have used a 60Co gamma-ray source with dose rate of 12.5 mGy/s. Whole blood from 15 healthy donors was collected. Whole blood from each donor was divided equally into 8 parts for exposing to supposedly physical doses 0, 0.30, 0.50, 1.00, 1.50, 2.00, 3.00 and 4.00 Gy for a independent calibration curve. Whole blood from 15 donors was used to calibrate dose – effect and statistical for general calibration curve. Using Poisson test (U-test) for the distribution of dicentric chromosomes in the metaphases to determine the uniformity of the radiation field. The average from 15 independent calibration curves of linear correlated coefficient was determined to be r (y, d) = 0.5136 ± 0.0038. The model equation derived is y = aD + bD2 + C. The calibration equation of dose-effect was y = 1.01D + 4.43D2 + 0.56.


How to cite this article:
Que T, Duy PN, Luyen BT. Calibration curve for dicentric chromosomes induced in human blood lymphocytes exposed to gamma rays at a dose rate of 12.5 mgy/s.Genome Integr 2016;7:2-2


How to cite this URL:
Que T, Duy PN, Luyen BT. Calibration curve for dicentric chromosomes induced in human blood lymphocytes exposed to gamma rays at a dose rate of 12.5 mgy/s. Genome Integr [serial online] 2016 [cited 2024 Mar 19 ];7:2-2
Available from: https://www.genome-integrity.org/article.asp?issn=2041-9414;year=2016;volume=7;issue=1;spage=2;epage=2;aulast=Que;type=0