Home
About us
Editorial board
Browse Articles
Submit article
Instructions
Subscribe
Contacts
Login
Advanced Search
Users Online: 168
» Articles published in the current year
To view other articles click corresponding year from the navigation links on the left side.
All
|
Case Report
|
Case Reports
|
Editorial
|
Erratum
|
Original Article
|
Original Articles
|
Review Articles
Export selected to
Endnote
Reference Manager
Procite
Medlars Format
RefWorks Format
BibTex Format
Show all abstracts
Show selected abstracts
Export selected to
Add to my list
Original Article:
Radiation-induced Chromosome Instability: The role of dose and dose rate
Eman Mohammed Elbakrawy, Mark A Hill, Munira A Kadhim
Genome Integr
2019, 10:1 (1 January 1900)
DOI
:10.4103/genint.genint_5_19
Nontargeted effects include radiation-induced genomic instability (RIGI) which is observed in the progeny of cells exposed to ionizing radiation and can be manifested in different ways, including chromosomal instability and micronucleus (MN) formation. Since genomic instability is commonly observed in tumors and has a role in tumor progression, RIGI has the potential of being an important mechanism for radiation-induced cancer. The work presented explores the role of dose and dose rate on RIGI, determined using a MN assay, in normal primary human fibroblast (HF19) cells exposed to either 0.1 Gy or 1 Gy of X-rays delivered either as an acute (0.42 Gy/min) or protracted (0.0031 Gy/min) exposure. While the expected increase in MN was observed following the first mitosis of the irradiated cells compared to unirradiated controls, the results also demonstrate a significant increase in MN yields in the progeny of these cells at 10 and 20 population doublings following irradiation. Minimal difference was observed between the two doses used (0.1 and 1 Gy) and the dose rates (acute and protracted). Therefore, these nontargeted effects have the potential to be important for the low-dose and dose-rate exposure. The results also show an enhancement of the cellular levels of reactive oxygen species after 20 population doublings, which suggests that ionising radiation (IR) could potentially perturb the homeostasis of oxidative stress and so modify the background rate of endogenous DNA damage induction. In conclusion, the investigations have demonstrated that normal primary human fibroblast (HF19) cells are susceptible to the induction of early DNA damage and RIGI, not only after a high dose and high dose rate exposure to low linear energy transfer, but also following low dose, low dose rate exposures. The results suggest that the mechanism of radiation induced RIGI in HF19 cells can be correlated with the induction of reactive oxygen species levels following exposure to 0.1 and 1 Gy low-dose rate and high-dose rate x-ray irradiation.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Sword Plugin for Repository]
Beta
Erratum:
Erratum: Conventional Risk Factors, Telomere Length, and Ischemic Heart disease: Insights into the Mediation Analysis
Genome Integr
0, 0:0 (1 January 1900)
DOI
:10.4103/2041-9414.325873
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Sword Plugin for Repository]
Beta
Advanced Search
Month wise articles
Figures next to the month indicate the number of articles in that month
2022
March
[
1
]
2021
November
[
1
]
May
[
1
]
2020
August
[
1
]
2019
October
[
1
]
July
[
1
]
May
[
1
]
2018
May
[
2
]
2017
January
[
7
]
2016
December
[
12
]
2015
September
[
1
]
April
[
2
]
1900
January
[
2
]
Sitemap
|
What's New
Feedback
|
Copyright and Disclaimer
|
Privacy Notice
© Genome Integrity | Published by Wolters Kluwer -
Medknow
Online since 29
th
November, 2014